Targeted and untargeted metabolomics for biomarker discovery, mechanism and translational research. BioTree's AQ1100 panel covers more than 1,100 targeted metabolites; NGM 2 Pro reports over 1,000 Level-1 metabolite identifications.
A broad panel covering more than 1,100 metabolites across 20 classes, plus focused assays for bile acids, SCFAs, hormones, neurotransmitters and more. BioTree states that a mixed standard containing the listed metabolites is tested alongside samples.
Untargeted profiling on an Orbitrap, using a combined multi-column strategy and BioTree's in-house library and algorithms to support higher-confidence metabolite identification.
BioTree lists the analytical platform, metabolite library and quality-control procedures for each metabolomics service.
Broad coverage in one AQ1100 workflow may reduce the need for multiple separate class assays, depending on the analytes required.
BioTree reports more than 1,000 Level-1 identifications for NGM 2 Pro. Annotation confidence should be considered when interpreting findings.
BioTree reports more than 3,500 reliably identified metabolites; the number observed in a project depends on the sample matrix and study design.
In a BioTree-cited microbiota-metabolite study, 21% of identified compounds had been overlooked by conventional non-targeted peak detection.
The figures below are BioTree's public AQ1100 specifications. Detection counts vary by sample type and study conditions.
Coverage is a starting point. A broad panel may reduce the need for multiple separate assays when the required analytes span several classes.
Reported detections, with context. BioTree reports an average of 600+ detections across the displayed human serum, stool, urine and mouse-liver samples; actual counts vary by matrix.
Two chromatographic conditions. BioTree uses Z-HILIC and C18 LC conditions to cover polar and non-polar metabolites.
Two questions. No email required. Review the listed service information based on your selections.
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Explore metabolites associated with differences between cohorts, then consider targeted follow-up where the study objective and assay scope support it.
Central carbon metabolism, including glycolysis, the pentose phosphate pathway and the TCA cycle.
Analysis of short-chain fatty acids, bile acids, indoles, TMAO and other gut microbiota-related metabolites.
Applications include pharmacological research and toxicity evaluation.
Applications include food nutrition and environmental toxicology.
Minimum biological replicates: n ≥ 30 per group.
BioTree cites a study in which metabolomic analysis identified indole-3-propionic acid as a key protective metabolite in a mouse cardiotoxicity model.
BioTree reports more than 1,000 Level-1 metabolite identifications and more than 3,500 reliable metabolite detections for NGM 2 Pro.
A BioTree-cited study annotated 672 microbiota-related metabolites; 21% had been overlooked by conventional non-targeted peak detection in that study.
Please request the source publication and current service documentation before relying on an example for study design.
Tell us the biological question, the sample type and roughly how many samples. We'll come back with a recommended workflow — and say so plainly if a cheaper approach would answer it.
AQ1100 uses a triple-quadrupole LC-MS platform and a broad panel of more than 1,100 metabolites. A focused targeted method may be more suitable when your question is limited to a defined analyte class.
AQ1100 covers 20 metabolite classes, including key pathways, microbiota-associated metabolites and published biomarkers. BioTree lists 100 µL plasma or serum as the minimum sample requirement; confirm suitability for your analytes and matrix before collection.
A focused targeted method may be more suitable when candidates cluster in a defined class or pathway. Confirm analyte coverage, sample requirements and quantification options before collection.
AQ Gut Flora 300 covers ten major categories of gut-related substances. In one BioTree-cited study, some identified microbiota-related metabolites had been overlooked by conventional non-targeted peak detection.
Glycolysis, PPP and TCA intermediates for energy-metabolism, cancer-metabolism and mitochondrial studies.
Defined signalling-molecule classes that may benefit from a focused targeted method; confirm limits and coverage for your matrix.
BioTree lists the QTRAP 6500+ platform and Z-HILIC and C18 chromatographic separation methods for AQ1100.
The listed categories include short-chain fatty acids, bile acids, indoles and trimethylamine-related metabolites.
A targeted service covering metabolites associated with central carbon metabolism.
Working with a matrix that isn't listed — CSF, milk, exosomes, culture medium or an unusual tissue? Please confirm feasibility and sample requirements before collection.
Untargeted profiling can produce many detected features with different levels of identification confidence. NGM 2 Pro is designed to support broader, higher-confidence metabolite identification; results vary by sample matrix and study design.
BioTree reports more than 1,000 Level-1 metabolite identifications for NGM 2 Pro.
Platform-reported Level-1 identification accuracy, up to 98.5%.
BioTree reports more than 3,500 reliably identified metabolites; observed results vary by project.
BioTree reports that up to 60% of metabolites may be consistently detected across cohorts at Level 1.
Untargeted datasets can include compounds reported at different annotation-confidence levels. Those levels should be considered when results are interpreted, reported and selected for follow-up.
BioTree reports more than 1,000 Level-1 metabolite identifications for NGM 2 Pro. Request the current technical documentation and annotation criteria for the exact project before making downstream decisions.
No single column retains the whole metabolome. BioTree states that NGM 2 Pro uses a combined multi-column strategy covering polar and non-polar metabolites, plus polar and non-polar lipids.
BioTree states that it has developed metabolite libraries and algorithms for more than a decade, and reports 96 patents and software copyrights across the company.
BioTree lists the Orbitrap Exploris 120 as the NGM 2 Pro platform. Its primary analytical targets are small molecules below 1,500 Da.
BioTree describes a stringent 9D quality-control system for NGM 2 Pro. Confirm project-specific QC documentation and acceptance criteria at quotation.
BioTree lists the following minimum numbers of biological replicates per group.
Per group, biological replicates, each analysed independently.
If your study has no defined candidate list, untargeted metabolomics may be appropriate. A targeted method may then be considered for follow-up, depending on the study objective and required quantification.
Deliverables are confirmed in the project quotation and may include data tables, quality-control information and agreed analysis outputs. Confirm annotation, statistical and pathway-analysis scope before ordering.
NGM 2 Pro is used for untargeted profiling. Quantification type, reporting format and any concentration-based options should be confirmed for the selected service and project scope.
Method information available for your project should be confirmed with the final service documentation. Please ask about the platform, chromatography, quality-control and annotation information relevant to your study.
You should not need to be a mass-spectrometrist to buy mass spectrometry. Here is what the vocabulary means and why each piece changes the data you get back.
Measurement of a defined metabolite list using a method selected for those analytes. It is commonly considered when the study has a focused biological question.
We look across the metabolome without a prior list, then identify what changed. Best for discovery — when the interesting metabolite is one you wouldn't have thought to request.
A high-confidence identification category used in metabolomics. Ask for the platform's current annotation criteria and how each reported metabolite is classified.
Concentration reporting in defined units. Whether it is available, and how results can be compared, depends on the selected assay, standards and validation scope.
A comparison of relative signal or abundance between samples or groups within a study. It is commonly used for discovery-oriented analyses.
A mass-spectrometry platform commonly used for targeted analysis of defined compounds. BioTree lists the QTRAP 6500+ for AQ1100.
A high-resolution mass-spectrometry platform. BioTree lists the Orbitrap Exploris 120 for NGM 2 Pro untargeted metabolomics.
Reference compounds added to an analytical workflow to support quality control and quantification. Their use and scope depend on the selected service.
Quality-control samples are used to monitor analytical performance. Request the project-specific QC approach and documentation at quotation.
Reference collections used to compare detected signals with known metabolite information. BioTree states that it has developed metabolite libraries and algorithms for more than a decade.
Two chromatographic separation methods are used to cover polar and non-polar metabolites.
Categories used to communicate identification confidence. Ask which annotation criteria and confidence information will be provided for your project.
BioTree was established in 2012 and reports a 6,000 m²+ R&D and service centre.
Send your biological question, sample type, approximate sample count and service of interest.
The information submitted includes your selected service, sample type, approximate sample count and research question.